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flag turboid mammalian expression vector  (Addgene inc)


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    Addgene inc flag turboid mammalian expression vector
    Flag Turboid Mammalian Expression Vector, supplied by Addgene inc, used in various techniques. Bioz Stars score: 94/100, based on 18 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/turboid+expression+vector/pm40304068-42-0-7?v=Addgene+inc
    Average 94 stars, based on 18 article reviews
    flag turboid mammalian expression vector - by Bioz Stars, 2026-07
    94/100 stars

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    Correlation analysis between DFNA5 and relate genes and markers of immune cells in TIMER

    Journal: Cancer Cell International

    Article Title: DFNA5 regulates immune cells infiltration and exhaustion

    doi: 10.1186/s12935-022-02487-0

    Figure Lengend Snippet: Correlation analysis between DFNA5 and relate genes and markers of immune cells in TIMER

    Article Snippet: A pcDNA3.0-flag, pcDNA3.0-PDCD1-3x-myc and pcDNA3.0-CD274-3x-myc plasmid expression vector were obtained from our lab. A pcDNA3-based expression vector for TurboID was obtained from addgene(Addgene: #107169).

    Techniques:

    Correlation of DFNA5 expression with tumour-infiltrating lymphocytes in COAD (colon adenocarcinoma), LIHC (hepatocellular carcinoma), and LUAD (lung adenocarcinoma). A DFNA5 expression is significantly negatively related to tumour purity and has significant positive correlations with infiltrating levels of CD8 + T cells, CD4 + T cells, macrophages, neutrophils, and dendritic cells in COAD but not B cells. B DFNA5 expression has significant correlations with tumour purity and infiltrating levels of B cells, CD8 + T cells, CD4 + T cells, macrophages, neutrophils, and dendritic cells in LIHC. C DFNA5 expression is significantly negatively related to tumour purity and has significant positive correlations with infiltrating levels of B cells, CD8 + T cells, CD4 + T cells, macrophages, neutrophils, and dendritic cells in LUAD. D The increased content of Flag-DFNA5 plasmids co-transfected with 3x-myc-PDCD1 or 3x-myc-CD274 plasmid, the expression levels of FLAG, 3x-myc and β-actin were detected by western blot. The band grey value of the rightmost well of 3x-myc-PDCD1 or 3x-myc-CD274 relative to the corresponding beta-actin were set at 1.0. The other treatments were compared with them

    Journal: Cancer Cell International

    Article Title: DFNA5 regulates immune cells infiltration and exhaustion

    doi: 10.1186/s12935-022-02487-0

    Figure Lengend Snippet: Correlation of DFNA5 expression with tumour-infiltrating lymphocytes in COAD (colon adenocarcinoma), LIHC (hepatocellular carcinoma), and LUAD (lung adenocarcinoma). A DFNA5 expression is significantly negatively related to tumour purity and has significant positive correlations with infiltrating levels of CD8 + T cells, CD4 + T cells, macrophages, neutrophils, and dendritic cells in COAD but not B cells. B DFNA5 expression has significant correlations with tumour purity and infiltrating levels of B cells, CD8 + T cells, CD4 + T cells, macrophages, neutrophils, and dendritic cells in LIHC. C DFNA5 expression is significantly negatively related to tumour purity and has significant positive correlations with infiltrating levels of B cells, CD8 + T cells, CD4 + T cells, macrophages, neutrophils, and dendritic cells in LUAD. D The increased content of Flag-DFNA5 plasmids co-transfected with 3x-myc-PDCD1 or 3x-myc-CD274 plasmid, the expression levels of FLAG, 3x-myc and β-actin were detected by western blot. The band grey value of the rightmost well of 3x-myc-PDCD1 or 3x-myc-CD274 relative to the corresponding beta-actin were set at 1.0. The other treatments were compared with them

    Article Snippet: A pcDNA3.0-flag, pcDNA3.0-PDCD1-3x-myc and pcDNA3.0-CD274-3x-myc plasmid expression vector were obtained from our lab. A pcDNA3-based expression vector for TurboID was obtained from addgene(Addgene: #107169).

    Techniques: Expressing, Transfection, Plasmid Preparation, Western Blot